Amino acid sequence:
KAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRII P RHLQLAVRNDEELNKLLGRVTIAQGGVLPNIQSVLLPK
Compared to the Pubmed Xen H2A sequence (Accession # AAA49769), the Luger lab construct (Accession # CAD89676) contains the mutation: G99R. The insert is meant to be between NdeI and BamHI sites. However, the NdeI site is interrupted by the start codon
* Calculated by ExPASy-ProtParam: https://web.expasy.org/cgi-bin/protparam/protparam