Description:

• Human histone octamers consist of 2 each of recombinant human histones H2A, H2B, H3 and H4 K_c20me2 expressed in E. coli
• Histone H4 K20C was treated under conditions followed protocol from a published paper below*  to install analog of dimethyl Lys20 

SGRGKGGKGLGKGGAKRHRK_cme2VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRD AVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG

* Simon MD et al. The Site-Specific Installation of Methyl-Lysine Analogs into Recombinant Histones. 2007. Cell 128(5): 1003-1012. 

• MW: 108638.34 , Extinction coefficient: 44700 **

• Storage buffer: 50% refolding buffer, 50% Glycerol (Refolding buffer: 10 mM Tris pH 7.5, 2 M NaCl, 1 mM EDTA and 5 mM B-mercaptoethanol)

• Stroe at -20^oC 

 ** Calculated by ExPASy-ProtParam: https://web.expasy.org/cgi-bin/protparam/protparam