Description:

• Human histone octamers consist of 2 each of recombinant human histones H2A, H2B, H3 K27me3 C96S C110A and H4 expressed in E. coli

• Histone H3 K27C C96S C110A was treated under conditions followed protocol from a published paper below*  to install analog of trimethyl Lys27 

ARTKQTARKSTGGKAPRKQLATKAARKme3SAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLP FQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLAAIHAKRVTIMPKDIQLARRIRG ERA

* Simon MD et al. The Site-Specific Installation of Methyl-Lysine Analogs into Recombinant Histones. 2007. Cell 128(5): 1003-1012. 

• MW: 108570.18 , Extinction coefficient: 44700 **

• Storage buffer: 50% refolding buffer, 50% Glycerol (Refolding buffer: 10 mM Tris pH 7.5, 2 M NaCl, 1 mM EDTA and 5 mM B-mercaptoethanol)

• Stroe at -20^oC 

 ** Calculated by ExPASy-ProtParam: https://web.expasy.org/cgi-bin/protparam/protparam